Messenger RNA.
نویسندگان
چکیده
منابع مشابه
Isolation and translation of hemoglobin messenger RNA from thalassemia, sickle cell anemia, and normal human reticulocytes.
Human hemoglobin messenger RNA was isolated by sucrose gradient centrifugation from reticulocytes of patients having various hemolytic anemias. Using a messenger RNA-dependent cell-free system derived entirely from rabbit reticulocytes, the human hemoglobin messenger RNA has been translated and the products analyzed by carboxymethylcellulose column chromatography. Normal messenger RNA directs s...
متن کاملStudies on Liver Messenger Ribonucleic Acid.
Strong evidence has accumulated in recent years in favor of the following scheme of protein biosynthesis. DNA directs the formation of complementary strands of messenger RNA which possess the necessary genetic information to determine the sequential arrangement of amino acids in polypeptide chains (1, 2). The messenger RNA attaches itself to ribosomes to form a multiple ribosomal structure, the...
متن کاملIsolation and Translation of Hemoglobin Messenger RNA from Thalassemia , Sickle Cell Anemia , and Normal Human
A B S T R A C T Human hemoglobin messenger RNA was isolated by sucrose gradient centrifugation from reticulocytes of patients having various hemolytic anemias. Using a messenger RNA-dependent cell-free system derived entirely from rabbit reticulocytes, the human hemoglobin messenger RNA has been translated and the products analyzed by carboxymethylcellulose column chromatography. Normal messeng...
متن کاملDesign, simplified cloning, and in-silico analysis of multisite small interfering RNA-targeting cassettes
Multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of RNA interference (RNAi) cassettes. Although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. Here, we worked out a simple cloning stra...
متن کاملComparative studies on specific and nonspecific binding of transfer ribonucleic acid to ribosomes.
The binding of aminoacyl transfer ribonucleic acid @RNA) to ribosomes in the presence of corresponding messenger RNA (specific binding) is compared with that in the absence of messenger RNA (nonspecific binding). Specific binding is much more sensitive to tetracycline than nonspecific binding, and the former is similar to, but not identical with, the latter in its response to changes in Mg++ co...
متن کاملMaternal histone messenger RNA: detection by molecular hybridization.
A competition-hybridization assay has been developed for the majority of sequences present in embryonically synthesized histone messenger RNA. The assay permits the first direct demonstration of specific "maternal" messengers in unfertilized sea-urchin eggs. The molecular size of histone messenger RNA stored in the egg appears to be the same as that of histone messenger RNA synthesized by the e...
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ورودعنوان ژورنال:
- Science
دوره 196 4290 شماره
صفحات -
تاریخ انتشار 1962